Determination of Optimum Programmed Temperature for Fatty Acid Analysis of Chlorella Microalgae Extract Using GCMS Instrument
نویسندگان
چکیده
Suhu kolom Gas Chromatography-Mass Spectrometry (GCMS) merupakan salah satu variabel yang harus diperhatikan pada analisis senyawa kimia, agar terpisahkan dengan baik. Penelitian ini bertujuan untuk menentukan suhu terprogram optimum asam lemak menggunakan instrumen GCMS. Sampel digunakan berasal dari hasil ekstrak mikroalga Chlorella. Ekstrasi Chlorella dilakukan cara sonikasi selama 60 menit pelarut n-heksana. Variasi GCMS dilakukan, yaitu kenaikan masing-masing 5 OC/menit, 7 OC /menit, 9 12 /menit awal 80 dan akhir 270 OC. Jumlah sampel diinjeksikan sebanyak 0,2 µL. Data diolah membandingkan waktu retensi lama analisis. Ditemukan, kondisi pengukuran, OC/menit sampai 21,1 menit. Asam terkandung dalam palmitat, oleat stearat. Dengan metode pengukuran menjadi lebih singkat diharapkan dapat teridentifikasi ; Kata kunci: Analisis, GCMS, terprogram, lemak,
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ژورنال
عنوان ژورنال: UNESA Journal of Chemistry
سال: 2023
ISSN: ['2252-8180']
DOI: https://doi.org/10.26740/ujc.v12n1.p20-25